goat polyclonal antibody against cxcl13  (R&D Systems)

Journal: Cell and tissue research

Article Title: CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung.

doi: 10.1007/s00441-021-03552-2

Figure Lengend Snippet: Fig. 4 CXCL13 is expressed in two-thirds of the murine tracheal neuroendocrine cells. a, b Immunohistochemistry of tracheal whole mounts and the corresponding quantification of their immunoreactive cells; maximum intensity projec- tions of z-stacks of confocal optical sections. a Immuno- histochemistry with antibodies against CXCL13 (a) (green) and PGP9.5 (a′) (red), labeling single neuroendocrine cells and nerve fibers. CXCL13+/ PGP9.5+ cells are indicated by arrowheads; CXCL13−/ PGP9.5+ cells are indicated by ( <). Data points in the scatter plot (a‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of CXCL13+/ PGP9.5+ and CXCL13−/ PGP9.5+ cells (n = 2254 cells pooled from 5 tracheas). b Immunohistochemistry with antibodies against CXCL13 (b) (green) and CGRP (b′) (red), labeling single neu- roendocrine cells and nerve fibers. CXCL13+/CGRP+ cells are indicated by arrowheads; CXCL13−/CGRP+ cells are indicated by ( <); CXCL13+/ CGRP− cells are indicated by (*). Data points in the scatter plot (b‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of phenotypes (n = 2650 cells pooled from 5 tracheas)

Article Snippet: Floating sections were rinsed in PBS, and unspecific protein binding sites were saturated with 10% normal porcine serum in 0.005 M PBS for 1 h. Sections were incubated overnight with goat polyclonal antibody against CXCL13 (1:400 AF470, R&D Systems) or rabbit polyclonal antibody against αCGRP (1:20,000; T-4032, Peninsula Laboratories) followed by incubation for 1 h with peroxidase-conjugated pig anti-rabbit Ig (1:100; P0217, Dako, Santa Clara, USA) to detect CGRP or with biotinylated secondary donkey anti-goat IgG (1:400; 705–065- 147, Dianova) to detect CXCL13.

Techniques: Immunohistochemistry, Labeling

Journal: Cell and tissue research

Article Title: CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung.

doi: 10.1007/s00441-021-03552-2

Figure Lengend Snippet: Fig. 6 CXCL13 is less expressed in murine broncho- pulmonary solitary and clustered neuroendocrine cells. Immunohistochemistry of lung cryosections with antibodies against CXCL13 (orange) and CGRP (green) and the relative frequencies of immunoreactive phenotypes. a Solitary neuroen- docrine cell co-labeled with antibodies against CXCL13 and CGRP. b Solitary neu- roendocrine cell only labeled with antibodies against CGRP. c A cluster of neuroendocrine cells (neuroepithelial body) consisting of more than 7 cells, 2 of them are co-labeled with antibodies against CXCL13 and CGRP. d Pie chart shows percentages of CXCL13+/ CGRP+ and CXCL13−/CGRP+- immunolabeled cells in solitary neuroendocrine cells (n = 73 cells pooled from 5 animals). e Pie chart shows the percent- age of CXCL13+/CGRP+ and CXCL13−/CGRP+- immunolabeled cells in neu- roepithelial bodies (n = 1475 cells pooled from 5 animals)

Article Snippet: Floating sections were rinsed in PBS, and unspecific protein binding sites were saturated with 10% normal porcine serum in 0.005 M PBS for 1 h. Sections were incubated overnight with goat polyclonal antibody against CXCL13 (1:400 AF470, R&D Systems) or rabbit polyclonal antibody against αCGRP (1:20,000; T-4032, Peninsula Laboratories) followed by incubation for 1 h with peroxidase-conjugated pig anti-rabbit Ig (1:100; P0217, Dako, Santa Clara, USA) to detect CGRP or with biotinylated secondary donkey anti-goat IgG (1:400; 705–065- 147, Dianova) to detect CXCL13.

Techniques: Immunohistochemistry, Labeling, Immunolabeling