goat polyclonal antibody against cxcl13 (R&D Systems)

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R&D Systems goat polyclonal antibody against cxcl13

Fig. 4 <t>CXCL13</t> is expressed in two-thirds of the murine tracheal neuroendocrine cells. a, b Immunohistochemistry of tracheal whole mounts and the corresponding quantification of their immunoreactive cells; maximum intensity projec- tions of z-stacks of confocal optical sections. a Immuno- histochemistry with antibodies against CXCL13 (a) (green) and PGP9.5 (a′) (red), labeling single neuroendocrine cells and nerve fibers. CXCL13+/ PGP9.5+ cells are indicated by arrowheads; CXCL13−/ PGP9.5+ cells are indicated by ( <). Data points in the scatter plot (a‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of CXCL13+/ PGP9.5+ and CXCL13−/ PGP9.5+ cells (n = 2254 cells pooled from 5 tracheas). b Immunohistochemistry with antibodies against CXCL13 (b) (green) and CGRP (b′) (red), labeling single neu- roendocrine cells and nerve fibers. CXCL13+/CGRP+ cells are indicated by arrowheads; CXCL13−/CGRP+ cells are indicated by ( <); CXCL13+/ CGRP− cells are indicated by (*). Data points in the scatter plot (b‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of phenotypes (n = 2650 cells pooled from 5 tracheas)

Goat Polyclonal Antibody Against Cxcl13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+mouse+cxcl13/Mouse+CXCL13%2FBLC%2FBCA-1+Antibody/pm34762185-78-30-37
Average 99 stars, based on 85 article reviews

goat polyclonal antibody against cxcl13 - by Bioz Stars, 2026-09

99/100 stars

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1) Product Images from "CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung."

Article Title: CXCL13 is expressed in a subpopulation of neuroendocrine cells in the murine trachea and lung.

Journal: Cell and tissue research

doi: 10.1007/s00441-021-03552-2

Fig. 4 CXCL13 is expressed in two-thirds of the murine tracheal neuroendocrine cells. a, b Immunohistochemistry of tracheal whole mounts and the corresponding quantification of their immunoreactive cells; maximum intensity projec- tions of z-stacks of confocal optical sections. a Immuno- histochemistry with antibodies against CXCL13 (a) (green) and PGP9.5 (a′) (red), labeling single neuroendocrine cells and nerve fibers. CXCL13+/ PGP9.5+ cells are indicated by arrowheads; CXCL13−/ PGP9.5+ cells are indicated by ( <). Data points in the scatter plot (a‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of CXCL13+/ PGP9.5+ and CXCL13−/ PGP9.5+ cells (n = 2254 cells pooled from 5 tracheas). b Immunohistochemistry with antibodies against CXCL13 (b) (green) and CGRP (b′) (red), labeling single neu- roendocrine cells and nerve fibers. CXCL13+/CGRP+ cells are indicated by arrowheads; CXCL13−/CGRP+ cells are indicated by ( <); CXCL13+/ CGRP− cells are indicated by (*). Data points in the scatter plot (b‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of phenotypes (n = 2650 cells pooled from 5 tracheas)

Figure Legend Snippet: Fig. 4 CXCL13 is expressed in two-thirds of the murine tracheal neuroendocrine cells. a, b Immunohistochemistry of tracheal whole mounts and the corresponding quantification of their immunoreactive cells; maximum intensity projec- tions of z-stacks of confocal optical sections. a Immuno- histochemistry with antibodies against CXCL13 (a) (green) and PGP9.5 (a′) (red), labeling single neuroendocrine cells and nerve fibers. CXCL13+/ PGP9.5+ cells are indicated by arrowheads; CXCL13−/ PGP9.5+ cells are indicated by ( <). Data points in the scatter plot (a‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of CXCL13+/ PGP9.5+ and CXCL13−/ PGP9.5+ cells (n = 2254 cells pooled from 5 tracheas). b Immunohistochemistry with antibodies against CXCL13 (b) (green) and CGRP (b′) (red), labeling single neu- roendocrine cells and nerve fibers. CXCL13+/CGRP+ cells are indicated by arrowheads; CXCL13−/CGRP+ cells are indicated by ( <); CXCL13+/ CGRP− cells are indicated by (*). Data points in the scatter plot (b‴) represent mean values of counts in one trachea (n = 5 tracheas); mean and SEM are indicated. The pie chart shows the percentages of phenotypes (n = 2650 cells pooled from 5 tracheas)

Techniques Used: Immunohistochemistry, Labeling

Fig. 6 CXCL13 is less expressed in murine broncho- pulmonary solitary and clustered neuroendocrine cells. Immunohistochemistry of lung cryosections with antibodies against CXCL13 (orange) and CGRP (green) and the relative frequencies of immunoreactive phenotypes. a Solitary neuroen- docrine cell co-labeled with antibodies against CXCL13 and CGRP. b Solitary neu- roendocrine cell only labeled with antibodies against CGRP. c A cluster of neuroendocrine cells (neuroepithelial body) consisting of more than 7 cells, 2 of them are co-labeled with antibodies against CXCL13 and CGRP. d Pie chart shows percentages of CXCL13+/ CGRP+ and CXCL13−/CGRP+- immunolabeled cells in solitary neuroendocrine cells (n = 73 cells pooled from 5 animals). e Pie chart shows the percent- age of CXCL13+/CGRP+ and CXCL13−/CGRP+- immunolabeled cells in neu- roepithelial bodies (n = 1475 cells pooled from 5 animals)

Figure Legend Snippet: Fig. 6 CXCL13 is less expressed in murine broncho- pulmonary solitary and clustered neuroendocrine cells. Immunohistochemistry of lung cryosections with antibodies against CXCL13 (orange) and CGRP (green) and the relative frequencies of immunoreactive phenotypes. a Solitary neuroen- docrine cell co-labeled with antibodies against CXCL13 and CGRP. b Solitary neu- roendocrine cell only labeled with antibodies against CGRP. c A cluster of neuroendocrine cells (neuroepithelial body) consisting of more than 7 cells, 2 of them are co-labeled with antibodies against CXCL13 and CGRP. d Pie chart shows percentages of CXCL13+/ CGRP+ and CXCL13−/CGRP+- immunolabeled cells in solitary neuroendocrine cells (n = 73 cells pooled from 5 animals). e Pie chart shows the percent- age of CXCL13+/CGRP+ and CXCL13−/CGRP+- immunolabeled cells in neu- roepithelial bodies (n = 1475 cells pooled from 5 animals)

Techniques Used: Immunohistochemistry, Labeling, Immunolabeling

Blocking Assay:

Article Title: Induction of Colonic M Cells during Intestinal Inflammation
Article Snippet: Cryostat sections were treated with 0.5% Tween-20 in PBS (Thermo Fisher Scientific), washed 3× in 0.1%Tween/PBS and then blocked using 0.1% Tween-20 in casein solution (Thermo Fisher Scientific). .. Primary antibodies, goat anti-mouse CXCL13 (R&D Systems Minneapolis, MN), rat anti-mouse ER-TR7 (AbD Serotec, Raleigh, NC), rat anti-mouse GP2 (MBL, Woburn, MA), and biotin-conjugated Ly6G and B220 (eBioscience) were then added diluted in blocking solution, and samples were again washed. .. Secondary antibodies were Alexa Fluor 488 (donkey anti-rat; Life Technologies, Carlsbad, CA), Alexa Fluor 647 (donkey anti-goat {"type":"entrez-nucleotide","attrs":{"text":"A21447","term_id":"583542","term_text":"A21447"}} A21447 and chicken anti-rat; Life Technologies), and streptavidin Alexa Fluor 647 (Life Technologies).

Staining:

Article Title: Bcl6 controls meningeal Th17–B cell interaction in murine neuroinflammation
Article Snippet: .. Primary antibodies used for staining were rat anti-mouse B220-FITC or -AF488 (clone RA3-6B2, BD, dilution 1:100), rat anti-mouse B220 (unlabelled, clone RA3-6B2, eBiosciences, 1:50), rat antimouse TCR Vβ11-FITC or -APC (clone KT11, BioLegend, 1:100), rat anti-mouse CD4-APC (clone L3T4, eBioscience, 1:100), rat anti-mouse CD45.2 (clone 30G12, conditioned Medium, (37), undiluted), rabbit anti-mouse Laminin-111 ‘pan laminin’ (38), Serum, 1:500), goat anti-mouse CXCL13 (R&D Systems, 1:50), rat anti-mouse ER-TR7 (clone ER-TR7, Dianova, 1:100). ..

Article Title: Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema
Article Snippet: After blockade, the slides were incubated overnight at 25°C with biotin rat anti-CD45R (B220, Cat. #553092; BD Pharmingen, San Diego, CA, USA) to visualize B cells and goat anti-CD3ε to detect T cells (sc-1127; Santa Cruz Biotechnology). .. Additionally, we also stained lung sections with goat anti-mouse CXCL13 (AF470; R&D Systems, Minneapolis, MN, USA) and biotin rat anti-mouse CD21-CD35 (Cat. #123406; BioLegend), in combination with rat anti-mouse follicular dendritic cell (FDC; FDC-M1, Cat. #551320; BD Pharmingen) to detect FDCs and CXCL13-producing cells. .. Finally, the slides were incubated for 2 h at room temperature with Alexa Fluor 568 donkey anti-goat IgG (Thermo Fisher Scientific) and streptavidin, conjugated to either Alexa Fluor 594 or Alexa Fluor 488, and counterstained with ProLong Gold anti-fade with DAPI (Thermo Fisher Scientific).

Article Title: Genetic ablation of histone deacetylase 2 leads to lung cellular senescence and lymphoid follicle formation in COPD/emphysema
Article Snippet: After blockade, the slides were incubated overnight at 25°Cwith biotin rat anti-CD45R (B220, Cat. #553092; BDPharmingen,SanDiego,CA,USA) tovisualizeBcells andgoat anti-CD3e to detect T cells (sc-1127; Santa Cruz Biotechnology). .. Additionally, we also stained lung sections with goat anti-mouse CXCL13 (AF470; R&D Systems, Minneapolis, MN, USA) and biotin rat anti-mouse CD21-CD35 (Cat. #123406; BioLegend), in combination with rat anti-mouse follicular dendritic cell (FDC; FDC-M1, Cat. #551320; BD Pharmingen) to detect FDCs and CXCL13-producing cells. .. Finally, the slideswere incubated for 2 h at room temperature with Alexa Flour 568 donkey anti-goat IgG (Thermo Fisher Scientific) and streptavidin, conjugated to either Alexa Fluor 594 or Alexa Fluor 488, and counterstained with ProLongGold anti-fadewithDAPI (Thermo Fisher Scientific).

Immunofluorescence:

Article Title: IL-22-Independent Protection from Colitis in the Absence of Nkx2.3 Transcription Factor in Mice.
Article Snippet: .. Immunofluorescence was performed as previously described (10) using the following commercially available Abs: goat anti-mouse CXCL13 (R&D Systems) developed with donkey anti-goat FITC (SouthernBiotech), biotinylated CD11c (clone N418; BioLegend) developed with Streptavidin PE (BD Biosciences), and biotinylated PNA (Sigma-Aldrich) developed with AMCA Streptavidin (Vector Laboratories). .. The following Ab clones were obtained from the American Type Culture Collection or produced in our laboratory, and then the Abs were purified and labeled in our laboratory: CD45-FITC (clone IBL-3/16), rat anti-mouse MAdCAM-1–AF555 (clone MECA-367), rat anti-mouse PNAd-Dylight594 (clone MECA-79, kindly provided by Dr. E. Butcher), B220-CF647 (clone 6B2), Thy-1–AF594 (clone IBL-1), and CD21/35-FITC (clone 7G6).

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